PURIFICATION AND CHARACTERIZATION OF THE CLONED HUMAN GM-CSF GENE EXPRESSED IN ESCHERICHIA COLI
نویسندگان
چکیده مقاله:
The human granulocyte-macrophage colony stimulation factor (hGM-CSF) gene was cloned in the pET 23a( +) expression vector under the control of strong bacteriophage T7 transcription and translation signals. The hGM-CSF gene was transferred into E. coli strainBL21 (DE3)pLysS andIPTG was used for induction of GM-CSF gene. Production of the target protein was obtained as revealed by ELISA and Western blot analysis. The produced 'h:GM-CSF was purified by immunoaffinity chromatography. The dot blot positive fractions were assayed for biological activity and it was shown that the expressed GM-CSF is active.
منابع مشابه
purification and characterization of the cloned human gm-csf gene expressed in escherichia coli
the human granulocyte-macrophage colony stimulation factor (hgm-csf) gene was cloned in the pet 23a( +) expression vector under the control of strong bacteriophage t7 transcription and translation signals. the hgm-csf gene was transferred into e. coli strainbl21 (de3)plyss andiptg was used for induction of gm-csf gene. production of the target protein was obtained as revealed by elisa and weste...
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عنوان ژورنال
دوره 12 شماره 4
صفحات 353- 357
تاریخ انتشار 1999-02
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